货号：E2284

储存条件：粉末-20°C可保存3年；液体-80°C可保存12月。

产品描述

ER (Estrogen Receptor) is the receptor of estrogens. ER is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors to mediate ERE-independent signaling. Equol is a metabolite produced in vivo from the soy phytoestrogen daidzein. Equol is a ligand for ER. According to a report, equol had 13 fold higher binding affinity preferential for ERβ than ERα, with Ki of 16 nM as detected by binding assays. Other data suggested that equol stimulated the growth of ER positive MCF-7 cells in a concentration-dependent manner when used between 10 nM to 10 μM. However, equol inhibited the growth of human prostate cancer LnCaP, DU145 and PC3 cells, with IC50s of 169 μM, 140 μM and 119 μM, as detected at 72h time point by MTT method. In PC3 cells, equol caused cell cycle arrest in the G2/M phase at the concentration of 150 μM and induced apoptosis at the concentration of 100 μM when incubated for 48h. In a PC3 xenograft model established in Balb/c nude mice, equol intragastricly administrated at the dose of 10 mg/kg daily for 33 days inhibited tumor growth by 43.2% as compared to control group.

产品信息