Dibutyryl-cAMP

货号：D0499

储存条件：粉末-20°C可保存3年；液体-80°C可保存12月。

产品描述

PKA is a ubiquitous cellular kinase, also known as cAMP-dependent protein kinase (cAMP), and it is well-established that plays an important role in regulating several functions of cell processes, including regulation of glycogen, sugar, and lipid metabolism. Dibutyryl-cAMP is a cell-permeable PKA activator that functions by preferentially activating endogenous cAMP. In vitro assays, Dibutyryl-cAMP was found to significantly suppress TNF-a production in a dose-dependent manner, with IC50 values of 247, 28.9, and 25.4 μM, respectively in RAW264.7 cells. Additionally, rat hepatocytes were cultured with cAMP agonists dibutyryl-cAMP inhibits Nitric oxide synthesis and inducible nitric oxide synthase (iNOS) expression through effects on the iNOS promoter region and NF-kB-binding activity. In hepatocytes, Dibutyryl-cAMP can prevent tumor necrosis factor α plus acti-nomycin D (TNFα/ActD)-induced apoptosis via a PKA-dependent mechanism. In vivo model, Dibutyryl-cAMP showed a distinct anti-fibrotic effect via PKA/p-CREB/CBP signaling. Treatment of Dibutyryl-cAMP resulted in the decrease of the number and size of silicosis nodules, inhibition of myofibroblast differentiation, and extracellular matrix deposition. Moreover, 4 days intra-peritoneal injections of bucladesine (600 nM/mouse) significantly reversed zinc chloride- and led acetate-induced avoidance memory retention alterations compared to control animals.

产品信息