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  • LabPAGE 4-20%  12 Wells 蛋白预制胶 (货号:P42012)
  • LabPAGE 4-20% 12 Wells 蛋白预制胶 (货号:P42012)

    • ¥320.00
      ¥320.00
CAS:


存储温度:4°C

凝胶选择


注:使用预制胶时,请务必按照以上配方配置电泳缓冲液,推荐使用本公司预制胶专用电泳液,MOPS-SDS速溶颗粒(货号:T7205),电泳缓冲液反复使用次数建议不超过3次。

产品简介

◆ 本产品为聚丙烯酰胺电泳凝胶,用于蛋白分离,单片胶为 12孔或 15 孔,12孔胶每孔 最大上样量为 50 uL,推荐最大上样量25 uL以下,15 孔胶每孔最大上样量为 30 uL,推荐最大上样量15 uL以下。

◆ 采用全自动凝胶灌注技术,产品的重复性好,质量稳定。独特的凝胶缓冲配方使蛋白

电泳条带更为清晰锐利,更加均匀,分辨率更高。

 ◆ 配套缓冲液为中性缓冲液,可以提高凝胶稳定性和避免蛋白在电泳过程中的再修饰。

产品特点

l 即开即用:无需自己配制各种溶液;

l 节省时间:最快20min跑完电泳;

l 安全放心:无需接触有毒试剂;

l 结果稳定:通过全自动、大规模的生产,保证每片胶之间良好的重复性,质量稳定;

l 条带完美:独特的设计,大大降低了边缘效应,电泳结果更完美;

l 性价比高:国货精品,质优价廉;

l 分辨率高:独特的凝胶缓冲配方使 蛋白电泳条带更清晰锐利,分辨率更高;

l 最佳搭配:搭配LABLEAD的Tris-MOPS-SDS速溶颗粒使用效果更佳。

预制胶使用简介

1) 缓冲液准备:取出一包Tris-MOPS-SDS速溶颗粒溶解于1 L去离子水中。

2)将预制胶从包装袋中取出,并撕去胶板底部的蓝色胶条。(如图1所示)

3)按箭头方向将梳子从胶板中平稳地平行推出。(如图2所示)


4) 推出梳子时,尽量避免孔道内有残留液体。

5)Bio-Rad、WIX等品牌硅胶密封条凸起的电泳槽(见图3)的使用注意事项:将电泳槽内框架的绿色硅胶密封条取出,然后将其平坦的一面朝外并重新插回内框架的凹槽中。(如图3所示)

6)装胶。(按照图4、图5所示的方法将胶板安装到凝胶电泳装置中)

(注意:务必确保将底部开口处置于胶条以下位置,不能被胶条遮挡。)

向电泳槽的内槽中倒入足够的Tris-MOPS-SDS电泳缓冲液,使其覆盖上样孔5-7 mm,在外槽中加入相同的电泳缓冲液以确保适当的冷却。为了获得最好的效果,外槽的缓冲液需要比内槽的位置稍低,不可漫过胶板。使用注射器或其他工具吸取适量1x的电泳缓冲液,将上样孔轻轻冲洗干净,去除气泡和残留的储存缓冲液。将蛋白质样品上样、电泳。

(推荐电压为160 V,最高不超过180 V。)

(注意:Tris-Glycine电泳缓冲液与LabPAGE™蛋白预制胶的Bis-Tris缓冲系统不兼容,请不要使用。)


8) 从胶板中取出凝胶。(如图6所示)

a、 电泳结束后,从电泳槽中将胶板取出。

b、 将合适的撬具小心插入胶板之间的空隙中。

C、按照图中所示方法慢慢撬动胶板上、中、下三个位置,直至胶板两侧完全分开。

d胶板打开之后,凝胶可能粘在任意一侧,将有凝胶的胶板有胶一侧浸入水中,贴着水面,胶板倾斜轻轻提起,凝胶掉入水中后,将凝胶从水中取出进行后续实验。


产品信息

LABLEAD

P00812

LabPAGE 8% 12 Wells(蛋白预制胶)

10块/盒

320.00

LABLEAD

P00815

LabPAGE 8% 15 Wells(蛋白预制胶)

10块/盒

320.00

LABLEAD

P01012

LabPAGE 10% 12 Wells(蛋白预制胶)

10块/盒

320.00

LABLEAD

P01015

LabPAGE 10% 15 Wells(蛋白预制胶)

10块/盒

320.00

LABLEAD

P01212

LabPAGE 12% 12 Wells(蛋白预制胶)

10块/盒

320.00

LABLEAD

P01215

LabPAGE 12% 15 Wells(蛋白预制胶)

10块/盒

320.00

LABLEAD

P41212

LabPAGE 4-12% 12 Wells (蛋白预制胶)

10块/盒

320.00

LABLEAD

P41215

LabPAGE 4-12% 15 Wells (蛋白预制胶)

10块/盒

320.00

LABLEAD

P42012

LabPAGE 4-20% 12 Wells (蛋白预制胶)

10块/盒

320.00

LABLEAD

P42015

LabPAGE 4-20% 15 Wells (蛋白预制胶)

10块/盒

320.00


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