货号：S9363

储存条件：粉末-20°C可保存3年；液体-80°C可保存12月。

产品描述

SN-38, a metabolite of CPT-11, is a semisynthesized derivative of camptothecin with much stronger cytotoxicity and single-strand breaks in vitro than CPT-11, which inhibits Topoisomerase I with IC50 values of 0.74μM and 1.9μM for inhibition of Topoisomerase I activity of P388 cells and Ehrlich cells, respectively. SN-38 inhibited topoisomerase I-induced relaxation of supercoiled DNA dose-dependently at concentrations ranging in 0.1-10μM. P388 cells exposure to SN-38 at concentration ranging in 0.01-1μM exhibited dose-dependent inhibition of DNA synthesis (IC50 of 77nM, 2-hour incubation) and RNA synthesis (1.3μM, 3-hour incubation), but no alteration in protein synthesis. Dose-dependent DNA single-strand breaks can also be observed in P388 cells treated with 0.5-2μM for 1h, without obvious double-strand breaks. CPT-11 is converted to SN-38 more efficiently in mice, but shows low efficiency on conversion into SN-38 in patients due to the difference in metabolic pathway.

产品信息